Sterile workshop management system

1. General Sterile workshop

1.1 The sterile workshop should be located on a dry floor. Good lighting and fluorescent lamps should be installed. The sterile workshop should have double-layer sealed windows, equipped with 48-inch ultraviolet lamps, and 1-1.2 meters above the table.

1.2 There should be a buffer room outside the sterile workshop, which must be equipped with ultraviolet lamps, etc., and a wash basin inside, with disinfectants, disinfection storage tanks, slippers and coat hooks.

1.3 The sterile workshop should have a mobile transfer window in the buffer room, air conditioning, thermometer, hygrometer and telephone.

1.4 The sterile workshop should be equipped with specimen cylinders or barrels filled with disinfectant and disinfectant cylinders and gauze for wiping the table.

1.5 Before the experiment, wipe the work table with iodine, place the required experimental equipment and various solutions, and turn on the ultraviolet lamp for disinfection for 1 hour.

(Figure 1: General sterile workshop)

1.6 Wash your hands in the buffer room before entering the sterile workshop, wear disinfection isolation clothing, slippers, masks, hats, and gloves when necessary.

1.7 Clean the used experimental equipment after the experiment. Equipment contaminated with germs must be boiled and disinfected for more than half an hour before washing.

1.8 The tabletop must be wiped and disinfected with iodine after the experiment. All reagents and experimental supplies must be returned to their original places. The experimenter should wash his hands with disinfectant and soap.

1.9 Change the isolation clothing and put it into the storage tank for high-pressure disinfection. Put the slippers worn in the sterile workshop back on the shoe rack. Boil the masks and hats after use, clean them, and then disinfect them with high pressure for standby use.

1.10 After using the sterile workshop, turn on the ultraviolet lamp for disinfection for 1 hour.

1.11 The sterile workshop and buffer room are cleaned once a week, and the slippers are cleaned once a week.

2. Strong poison room

2.1 Strictly start and shut down according to standard operating procedures.

2.2 To Enter the clean room for work, you must go through the air shower, wear sterilized isolation clothing, hats, masks and gloves.

2.3 After the experiment, waste, laboratory equipment, isolation clothing, caps, masks, etc. should be disinfected at 121℃ for 1 hour before they can be removed from the room for cleaning or disposal.

2.4 The operating table in the toxicology room should be rubbed with disinfectant after operation, and the staff should rub their hands with disinfectant and then wash them with soap and water.

2.5 The toxicology room and buffer room should be cleaned once a week.

(Figure 2: Toxicology Room)

2.6 The slippers used in the toxicology room should be cleaned once a week.

2.7 Conventional disinfectants and effective disinfectants for emergency accidents are available in the room.

2.8 Eating, drinking, chatting and smoking are strictly prohibited in the laboratory.

2.9 The door of the toxicology room should be locked in time after use.

2.10 The pressure cooker and the Clean bench should be regularly quality controlled and records should be kept.

2.11 The Wind speed and air intake and exhaust volume of the air inlet and exhaust ports should be measured regularly.

2.12 The dust concentration and bacterial colony count in the clean room air should be measured regularly.