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What is a Horizontal Laminar Flow Hood?
A horizontal laminar flow hood is a specialized workspace designed to provide a sterile environment by directing hepa-filtered air horizontally across the work surface, protecting samples from contamination.
The primary function is to create a controlled airflow that minimizes exposure to airborne contaminants. The filtered air flows over the workspace, ensuring that any particulates are swept away from sensitive materials.
Structure & Design
Horizontal laminar flow hoods consist of:
- A sturdy cabinet with a transparent front sash
- A work surface made of easy-to-clean materials
- An airflow system with pre-filters and hepa filters
This design provides both visibility and accessibility while maintaining sterility.
airflow design & Low Turbulence
The airflow design is critical to maintaining a sterile environment:
- Air is pulled through a pre-filter and HEPA filter
- Filtered air is uniformly distributed across the work surface
- Uniformity minimizes turbulence and backdrafts
Low turbulence ensures a smooth, consistent layer of Clean air, reducing the likelihood of contaminants settling on samples.
Scope of Use
| Field | Applications |
|---|---|
| Microbiology Labs | Culturing microorganisms, handling biological samples |
| Cell Culture | Growing cells, tissues, and organoids |
| Pharmaceutical Companies | Compounding sterile medications |
Not Suitable for Hazardous Materials
Key Limitations:
- User Safety: Inadequate protection against toxic substances
- Contamination Risks: Airflow directed towards the user may expose operators to hazards
- Standards Compliance: Not suitable for applications requiring biosafety standards
Maintenance & Standards
Routine Maintenance:
- Inspect hepa filters, airflow velocity, and workspace cleanliness
- Replace filters every 6-12 months (depending on usage)
- Clean interior surfaces with appropriate disinfectants
Key Standards:
- ISO 14644 (CleanRoom Classification)
- NSF/ANSI 49 (Biosafety Cabinets)
- IEST and ANSI standards for performance testing
Summary
A horizontal laminar flow hood is a critical laboratory equipment that provides a clean and sterile working environment by directing filtered air horizontally across the workspace. This unidirectional airflow prevents contamination, making it ideal for high-cleanliness applications in microbiology, pharmaceuticals, and research.
